Infertility Research Today is a free monthly online journal that collates and summarizes the latest research about Infertility, including details on male and female infertility, treatment, causes, pregnancy. | ||||||||
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Adequate ovarian follicular status does not prevent the decrease in pregnancy rates associated with high sperm DNA fragmentation.Frydman N, Prisant N, Hesters L, Frydman R, Tachdjian G, Cohen-Bacrie P, Fanchin R Department of Genetics and Reproduction, Antoine Béclère Hospital, Clamart, France. nelly.frydman@abc.ap-hop-paris.fr OBJECTIVE: Potential reparation of sperm DNA fragmentation in the oocyte may disturb any relationship between DNA-damaged sperm and the implantation ability of resulting embryos. To rule out this factor, we analyzed the consequences of sperm DNA fragmentation on IVF-ET outcome in women with healthy ovarian function. DESIGN: Prospective study. SETTING: Teaching hospital, France. PATIENT(S): All 117 women were <38 years old, who combined normal serum day-3 FSH and inhibin B levels with an adequate response to controlled ovarian hyperstimulation. INTERVENTION(S): The DNA fragmentation rate was determined in the raw sperm used for conventional IVF by flow cytometric terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling assay. Cycles were sorted into two groups according to whether DNA fragmentation exceeded (high fragmentation [HF], n = 52) or did not exceed (low fragmentation [LF], n = 65) the 50th percentile of values (35%). MAIN OUTCOME MEASURE(S): D2 embryo quality and implantation and ongoing pregnancy rates. RESULT(S): Patients' characteristics, raw semen parameters, fertilization rates, and embryology data were similar in HF and LF groups. Clinical (37.5% vs. 62.5%) and ongoing (23.5% vs. 57.8%) pregnancy rates per ET and implantation rates (24.5% vs. 42.4%) were lower in the HF group than in the LF group. CONCLUSION(S): High sperm DNA fragmentation spares fertilization and top embryo morphology rates but is associated with decreased IVF-ET outcome. Published 14 January 2008 in Fertil Steril, 89(1): 92-7.
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